Cattle

Cattle are one of the most used animals for science in NZ. Learn about the many ways they are used!

Thousands of cattle are used for scientific purposes in Aotearoa every year. In fact, they have been one of the most commonly used animals in NZ science since 1989!

This sadly makes sense when you consider how widely they are exploited for their milk and their bodies.

The species of cattle is simplified to cows by many people. Actually, only the mothers are called cows:

  • Heifers are female cattle before they become mothers.
  • Calves are their babies.
  • The males are referred to as bulls (or steers if castrated).
Animal agriculture:

Thousands of cattle are used in Aotearoa every year in research aimed at trying to sustain, enhance and make more money for the animal agriculture industry.

Examples include research into:

1. Decreasing their negative impact on the environment - a cow on its own won't create a huge impact but because we have bred so many cattle in NZ, their collective impact is huge!

  • Cattle have been put in respiration chambers so the amount of methane they produce can be measured. Researchers have also glued urine sensors around their vulva to try and investigate ways of reducing the amount of nitrogen produced in their urine (methane and nitrogen can create negative impacts on the environment).

2. Increasing the survival rate of calves - not for the calves themselves but so the animal agriculture industry doesn't lose as much money.

3. Learning more about the fertility of cows (i.e., trying to improve artificial insemination and reduce the rest time in-between pregnancies for cows). Again, this research is not done for the cows themselves but so they be made pregnant as often as possible so that more money can be made from their exploitation.

4. Genetic modification to try and create more desirable traits in cattle (i.e., genetically modifying cows to change the properties of their milk).

5. Diseases that cattle on farms often get such as lameness, mastitis (a common infection of the udder in dairy cows) and fungal and parasitic infections. This type of research often involves making the animals sick on purpose.

6. Ways of housing and keeping large numbers of cattle on farms (i.e., research into bedding, floor types, shelter, shade and hygiene practices).

7. Trying to find more “humane” slaughter methods (i.e., investigating what type of gas kills piglets the quickest and if stunning animals before killing them creates less of a pain response).

8. Different types of pain relief for standard procedures in the animal agriculture industry (i.e. dehorning, castration, C-section).

9. Safety tests for chemicals and animal remedies, including medications, supplements and fertilizers.

10. Increasing milk or muscle (beef) production (i.e., feeding cattle different additives and measuring how its digested).

Fistulation: Cattle with holes cut into their bodies

For decades, researchers have artificially created fistulas (also known as cannulas) in ruminant animals such as cattle, sheep and deer. These are essentially window-like holes in the side of an animal's body.

Fistulas are tubes that are created surgically to connect a cow or sheep’s stomach to her skin. The outside of the fistula is covered with a plastic cap that can be opened by scientists to remove stomach contents for analysis or introduce foreign substances.

It is a painful procedure and cows are still suffering from the intrusive surgery up to 11 days after the surgery. Learn more here.

Additional calf exploitation - the calf blood industry

Calves and calf foetuses are killed, and the blood is drained from their bodies to be used in other research.

There are two main types of calf blood products: Fetal bovine serum and New Born Calf Serum.

Fetal bovine serum is taken from foetuses that have been removed from their mothers in slaughterhouses after they have been killed. A needle is injected into the foetus’ heart, and the blood is pumped out. The foetuses have to be alive during this so that their blood comes out easier. They also have to be at least three months into the gestation period to ensure that their heart is large enough to puncture.

Newborn Calf Serum is sourced similarly; calves who are 20 days old or younger have their heart punctured and are bled to death.

These products are used as a media base and growth supplement in many different types of research.

In the news

Mycoplasma Bovis: "Researchers will measure how M. Bovis impacts infected animals and herds, including physical signs, effects on milk yield and quality, weight gain in cattle, and the duration of these effects." Read more.

Cow shock collars: "Animals wear GPS-enabled collars that identify their location relative to invisible fence lines created on a digital farm map. The animals are trained to be guided by audio prompts (beeps) from the collars when required. As an animal gets close to a virtual fence, a series of beeps alerts them to the fence boundary. If they cross the fence line, they receive a "disincentive", a mild electric shock, and are guided back inside the line." Read more.

Bobby calves: Bobby calves (male calves of dairy cows) are normally unwanted by the dairy industry. Researchers are cross-breeding dairy cows with beef breeds so that bobby calves can be killed and eaten after a year, rather than being killed as "excess" after 4 days. Read more

Climate change: AgResearch scientists have been given $10 million to develop cattle better adapted to warmer temperatures and to lower methane emissions. Read more.

Places that use cattle for science in NZ include universities such as Lincoln University (who have their own research dairy farm) and Massey University (they also have their own dairy farms), private companies such as Abacus Bio Limited and Cognosco, crown institutes such as AgResearch, industry bodies such as Dairy NZ and by other organisations and individuals.

Cattle used for science in NZ are sourced from commercial establishments, farms, public sources, breeding units and other sources.

Discover real-life examples of how cattle have been used in NZ below!
References:

Studying selenium metabolism

Cats and dogs were fed diets with different selenium supplementation for three weeks in individual metabolic chambers. A liver biopsy was taken under anaesthesia.

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Monitoring wild cats via GPS collar

Wild cats were caught and fitted with GPS collars. After an average of 9.75 days, there were recaptured to retrieve the collars.

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Testing new toxin against stoats and wild pets

Dogs, cats and stoats were fed different versions of toxic bait. Symptoms and time of death were monitored. Four dogs and two cats survived, but their fate is not stated.

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Testing the efficacy and shelf-life of a toxin

Captive wild cats were used to compare the efficacy of different toxin formulations and the effect of more extended bait storage. Cats were fed the toxin bait or pipette-fed toxin directly as control. Symptom onset and time of death were monitored.

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Studying the genes for hairyness

The hairiness of male and female cattle was rated. Some animals had their rectal temperature taken repeatedly, and some were shut in a hot room for two hours to make them sweat. Eight calves were killed to take brain samples.

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Optimising fertility treatment

Young female cattle were treated with different hormonal injections to control their fertility cycle. Around ten rectal ultrasounds were done on each of them within 11 days, and several blood samples were taken in the next month. All but nine were artificially inseminated. The nine animals showed no fertility signs and were therefore useless for the study and the industry.

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Trying out a health test for breeding bulls

Breeding bulls were infected with a skin fungus that usually results in a dangerous build-up in the gall and eczema. Through a special diet, bulls can be tested for their reactions via repeated blood samples and ranked for their genetic quality.

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Testing a vaccine against miscarriage

Younger and older pregnant cattle were injected with a new vaccine against a parasite or just control solution. Skin reactions to the injection were checked, and blood samples were taken. Farmers reported miscarriages when they happened, and dead foetuses were collected if possible. If female calves were born, blood samples were taken from them, too.

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Studying Johne’s disease

Some female calves of a group were infected with bacteria causing life-threatening sickness. Poo samples were collected directly from the rectum of all calves every other month. Half were killed to be dissected at 7 months, the rest at 15 months.

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Studying Johne’s disease bacteria

Blood samples were taken from sick dairy cows who were sent to slaughter to see if their immune system was even putting up a fight. After slaughter, organ samples were taken.

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Finding a gene to increase casein in milk

Breeding bulls were evaluated on their quality by judging, breeding, and milking their daughters. No exact numbers are stated how many lived to the end of the study (5 lactations).

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Testing a new way to treat liver flukes

Young cattle were infected with liver flukes. They were treated with different medications or left untreated. Regular blood samples were taken from all animals for three weeks, and they were killed ten weeks later.

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Studying effects of stunning before slaughter

Calves and sheep were anaesthetised. Some got 70-volt shocks to the shoulder. Others had a nerve exposed and stimulated before being stunned or cutting their throat.

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Measuring brain reactions to stunning after killing

Calves were fasted for 24 hours and then anaesthetised. They were killed by their throats being cut, followed by stunning while measuring EEG/ECG (electronic heart and brain activity) and blood pressure.

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Measuring brain reactions to stunning

Calves were fasted, anaesthetised and stunned with a non-penetrative bolt while being monitored for their response. After measurements were taken, they were killed.

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Studying brain reactions to cutting blood vessels or neck tissue

Calves were anaesthetised, and their throat blood vessels exposed. While monitoring the brain“s activity (EEG) and blood pressure, either only the blood vessels or only the neck tissue was cut. The latter animals were then killed, too.

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Measuring if being slaughtered is unpleasant

Calves were fasted, anaesthetised, and killed by cutting their throat. EEG and ECG (recording electronic heart and brain activity) were measured with electrodes in the head.

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Measuring brain reactions to pain

Twenty calves were fasted and anaesthetised. Their horn buds were removed with a scoop, and pain response of the heart and brain with and without pain blockers.

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Testing mastitis treatments

Dairy cows with an udder infection were treated with different antibiotic injections. Milk samples were taken to monitor results.

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Trying to clone cows with more valuable milk protein

The genetic material of a cow foetus was manipulated and cloned into several cows. When they were less than one year old, they were injected with hormones to produce milk for sampling.

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Analysing Greyhound race injury statistics

Historical data on Greyhound races between 2014 and 2019 were analysed. Injuries and deaths were investigated.

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Using bones of euthanised shelter dog

Cadavers of dogs killed at an animal shelter were donated. Holes were drilled into leg bones and fitted with screws.

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Investigating farm dogs for four years

Working farms dogs were examined regularly for four years. Health problems and fate were recorded.

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Transplanting faeces as diarrhoea treatment

A dog with diarrhoea was treated with laxatives and warm water before a faeces sample from a healthy dog was inserted into his colon. Samples were taken before and after.

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Testing the eyes of working dogs

Eye examinations were performed on working sheep dogs. Medication for pupil dilation was given, and faeces samples were taken from most dogs.

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Analysing dog movements

Colony dogs were prepared with reflective markers and an activity collar. Their movements on a treadmill at various speeds were recorded.

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Testing the effect of diet on farm dog performance

Working dogs were fed a diet very high or very low in carbohydrates. Their blood glucose and activity were monitored three times over four days each.

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Testing a parasite treatment

After a pilot study with just one dog, six more were given an experimental parasitic treatment. Regular blood samples were taken through a catheter.

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Teaching dogs to smell out catfish

Five pet dogs were trained over several months to identify catfish and goldfish smell in water samples.

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Testing different combinations of pain meds

Eight dogs are treated with four different combinations of the test drugs in random order with two-week breaks in between. Two catheters are placed in leg veins (for drug injection and blood samples). For the test, dogs are fasted for 12 hours and anaesthetised. EEG is recorded with electrodes near the brain to measure response to electric shocks. Regular blood samples are taken for 8 hours.

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Studying medical data of cat and dog CT-imaging

Medical data from cats and dogs were gathered, and animals that had been euthanised for other reasons were examined.

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Teaching dogs to smell out koi carps (2019)

Three pet dogs were trained over several months to identify koi carb smell in water samples.

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Testing working dogs for internal parasites

Faeces and blood samples were taken from working farm dogs.

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Testing fluid management of dogs during anaesthesia

Dogs were anaesthetised. Their heart activity was measured while hey were injected with calculated amounts of fluid.

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Trying to make rat poison unattractive for dogs

Dogs were fasted overnight and then given two food bowls with different kinds of repellent on one of them.

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Surveying respiratory disease in racing Greyhounds

Laboratories sent in serum samples of dogs for analysis. Additionally, 17 racing Greyhounds were tested.

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Studying fish behaviour in groups of multiple species

Mixed-species groups of fish (partly caught in the wild) were placed in a tank and fasted for 24 hours. Food was given and an artificial predator was used to study behaviour.

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Studying food chains near CO2 vents

Fish and snails were observed and counted near CO2 vents and at control sites. Fish were also caught to examine their stomach content.

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Studying fish behaviour in single-species groups

Young fish were caught in the wild and split into groups of 15, 75, and 150 animals. After being fasted for 24 hours, food was given and an artificial predator was used to study behaviour.

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Testing the side-bias of fish (if they prefer to swim left or right)

Wild-caught fish were kept for 1.5 years before each fish was placed on a runway to observe their behaviour. Afterwards, fish were observed for schooling movements and some were killed to dissect their brains.

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Studying effects of Climate Change on farming in damselfish

Fish were observed for their algae farming behaviour near CO2 vents and at control sites (Bay of Plenty). Small areas were covered to keep the fish away for a month and compare algae growths measured to compare.

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Trying to estimate habitat quality from body condition

Snappers were hatched from eggs and raised on commercial food. They were kept in 800-litre flow-through tanks containing 170 to 260 fish each. At 80 days, they were fasted killed.

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Trying different techniques for tagging fish

Fish were bait-trapped and treated for parasites. They were used to test different tagging methods. In the second part of the experiment, fish were caught with two different methods, after which injuries and survival over 12 days were monitored.

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Studying fish near volcanic vents to learn about Climate Change

Fish near volcanic CO2 vents and at control sites were observed and counted. The reaction to baited underwater cameras and predator threats was filmed. Aquarium experiments were conducted with caught fish of different species.

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Verifying “fish-friendly” flood pumps

Eels were caught, measured and tagged with transponders into the gut skin. They were later re-captured, observed for wounds and fungus and killed to examine internal damage.

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Testing the temperature tolerance of Antarctic fish

Wild-caught fish were kept individually for months in tanks. During temperature experiments, fish of each group were tested for their breathing ability in small tubes before all fish were killed.

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Slowly poisoning fish

Snappers were fasted for three days and then fed with toxic fish. Several died during feeding, and the rest were killed at the end.

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Studying population changes in reef fish

Over 1,000 fish were caught by spearing at three NZ locations.

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Testing the hearing of fish

Fish were anaesthetised and strapped to a plastic slide near an underwater speaker. Electrodes were placed in their head and gills to measure brain activity. For medical imaging, fish were restrained in a plastic tube with a sponge to keep them wet.

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Questioning vets on pain management

Veterinarians completed a questionnaire regarding pain and pain management in rabbits and guinea pigs.

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Testing nerve reactions to electric stimulation

Young guinea pigs were bled to death. Specific nerves were removed and experimented on.

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Studying short-sightedness

Guinea pigs had one eye covered for two weeks before increasing their eye pressure surgically and kill them.

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Measuring nerve reactions to gases

Guinea pigs were anaesthetised, and their windpipe and blood vessels were cannulated. They were exposed to different gases and cyanide while measuring breathing and nerve activity.

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Studying how anaesthesia affects breathing and heart

Guinea pigs were put in airtight chambers to test the effects of low oxygen. Some were surgically fitted with tubing so blood samples could be taken during the tests. In the end, all were killed.

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Studying compensation after inner ear destruction

Guinea pigs were anaesthetised, and their inner ear was destroyed with a drill. Videos were made for over two days of their balance recovery.

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Killing pregnant guinea pigs for tissue cultures

Pregnant guinea pigs were anaesthetised at specific time points of their pregnancy, including during labour, to then remove the uterus for tissue culture experiments.

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Testing how oxytocin injections cause contractions

Pregnant guinea pigs were surgically implanted with electrodes on or near the uterus. They were injected with oxytocin in specific doses and timeframes, and the effects were measured.

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Harvesting testicles for studies

Male guinea pigs were killed with CO2 to remove their testicles for experiments.

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Testing a new tuberculosis vaccine

A new tuberculosis vaccine was tested by injecting guinea pigs with the bacteria, either with or without prior vaccination. After several weeks, all were killed.

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Testing how heart calcium metabolism is different in species

Guinea pigs and rats were decapitated, and their hearts were quickly removed to study them.

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Testing influences on the rate of a dissected heart

Albino guinea pigs were anaesthetised and dissected before they were killed.

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Confirming the correlation of energy storage and stress in the inner ear fluid

Guinea pigs were anaesthetised and artificially ventilated. Ear fluid samples were taken through a hole in the skull, while exposing them to noise or lack of oxygen.

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Comparing natural and genetically altered tuberculosis strains

Guinea pigs were infected with tuberculosis strains after being injected with bacterial proteins beforehand. In the end, all guinea pigs were killed and dissected.

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Comparing effects of anaesthetics on breathing and heart rate

Guinea pigs, rabbits and rats were anaesthetised, and a tube was put into their windpipe through the neck. They were made to breathe anaesthetic gas.

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Researching scoliosis genes

Transgenic mice with a specific gene defect were mated. Pregnant mice were at several time points. The unborn babies were taken, skinned, and gutted to stain their skeletons for better pictures.

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Studying hormone influences on obesity and fertility

Transgenic mice were bred to have diabetes symptoms. Together with control mice, they were fed a normal or a high caloric diet and compared for their fertility over 150 days.

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Testing a new stroke treatment

Mice were squeezed into plastic cones, and test drugs were dripped on their nose. An artificial stroke was caused by injecting a chemical into their brain. Before and after, mice had to perform behavioural tests. All were then killed to take their brains.

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Testing another new stroke treatment

Mice were operated on several times. They were made to experience an artificial stroke, were later injected with a possible treatment, and then were injected with a marker. The mice had their memory tested before they were killed.

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Testing prostate cancer drugs

Mice were anaesthetised, and their prostate was injected with cancer cells or a control solution. A 42-day treatment course started three weeks later with different treatment drugs and control solutions. In the end, all mice were killed.

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Testing a heart attack treatment

Mice were trained to eat jelly and then subjected to an artificial heart attack. They received either a new drug or a control substance. Regular heart ultrasounds were performed before they were killed and dissected.

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Studying ovary problems caused by testosterone

Transgenic mice were implanted with a capsule containing either hormones or not. Vaginal smears were done for several weeks, and serial blood tests were performed twice. In the end, all mice were killed.

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Testing an Autism treatment on transgenic mice

Mice were genetically modified to show "autism-like" symptoms. Together with control animals, they were either fed normally or with a high-zinc diet. Invasive behavioural tests were performed. At least some, likely all, were killed.

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Comparing transgenic mice and humans

Mice were anaesthetised, and their body composition was measured several times. Their grip strength and balance were tested with lab equipment. In the end, all mice were killed.

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Testing a new stroke drug for better dexterity

Transgenic mice were anaesthetised, and an artificial stroke was caused. They then received either a test drug or a control solution. After recovery, their food was limited to test their fine motor skills every other week. After two months, they were killed.

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Testing migraine drugs

Mice were injected with potential migraine drugs and anaesthetised. Ultrasound imaging of their ears was taken. One ear was rubbed with capsaicin to record the increased blood flow and compare different drugs, doses, and time points.

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Killing rats and mice for tissue

Rats and mice were anaesthetised, and their necks were broken. Their tissue was then used for research.

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Studying Alzheimer’s and stroke symptoms

One group of transgenic mice were bred and killed at either four or 12 months of age by flushing their hearts. Another group was anaesthetised and caused to have a stroke. They were killed the same way.

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Testing Alzheimer’s treatment on mice

Transgenic and “normal” mice were injected with a special protein and a chemical to mark new brain cells that may have been produced.

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Testing a new Alzheimer’s drug

Newborn mice were killed to collect brain tissue. Adult mice were used to test drug reactions. They were anaesthetised twice; to inject the drugs and to flush their hearts and take their brains.

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Testing if anaesthesia affects the sleep–wake cycle when combined with light treatment

Mice were kept on a 12-hour light/dark cycle and then switched to total darkness. During this time, they were exposed to light once, either with or without anaesthesia. The effect on their activity cycles was monitored. In the end, all were killed.

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Studying the effect of certain lipids in formula on brain development

Piglets were kept individually and fed formula with different supplementation. After 18 days, they were killed.

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Harvesting bladders to study contraction patterns

The bladders of six pigs were surgically removed under anaesthesia before the pigs were killed.

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Testing toxic bait

Pigs were fed toxic bait and monitored until they died.

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Testing the effect of a shot to the head

Pigs were anaesthetised and shot in the back of the head from different distances. Effects were recorded and analysed.

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Training students in surgical procedures

Pigs and sheep were used for training future surgeons.

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Teaching students surgical techniques

Sheep and pigs were used and killed in teaching students.

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Testing the viability of xenotransplantation using islet capsules

Newborn piglets were killed for their pancreas. Encapsulated pancreas cells were then implanted into young mice, who were killed later to retrieve the capsules.

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Testing measurement of specific proteins

Pigs were surgically fitted with an outside access to their intestine and kept individually for eight weeks. Digestive matter was collected over ten hours on one day, while the pigs had no access to food or water.

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Studying the spread of bovine tuberculosis in pigs

Pigs were bought, and wild pigs were captured and distributed through two studies over almost two years. Pigs were killed regularly to dissect them for tuberculosis signs.

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Studying wound healing treatments

Pigs were anaesthetised and wounded in 20 places on their backs. The wounds were treated differently and sampled over a month before the pigs were killed.

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Developing a model for varicose veins

Blood vessels in pigs' legs were surgically altered, and some pigs were fitted with devices to measure blood pressure. After weeks of regular examinations, all were killed.

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Testing how long you have to wait to shoot and eat poisoned wild pigs

Piglets were fed different amounts of rat poison for longer or shorter periods before they were all killed.

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Testing how many cancer cells spill out during colon surgery

Pigs were anaesthetised and injected with radioactive cancer cells. Different colon surgeries were performed.

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Studying how ageing affects brain chemistry

Rats were kept in germ-free, individual cages before being killed. Their brains were used for tests.

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Studying teen binge drinking

Young rats were force-fed alcohol repeatedly. Each time, blood samples were taken, and some were killed to take their brains after each alcohol day. The remaining rats were put through behavioural tests a few months later.

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Teaching animal researchers

A range of animals is used to teach researchers handling and experimental methods.

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Testing medications for drug addiction

Rats were taught to inject themselves with cocaine when they pressed a lever. Through many cycles of establishment and withdrawal, medications were tested for their effect on drug-seeking behaviour. Other rats were tested for their activity level on cocaine with or without medication. Some were put into water-filled cylinders to examine if medication influences how long they swim.

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Testing effects of meth on brain protein

Rats were taught to inject themselves with meth or a saline solution when they pressed a lever. After 20 days, there were two weeks of abstinence. In the end, all were killed to dissect their brains.

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Testing new drug formulations

Rats and rabbits were injected with a new anaesthetic, pinching their paw repeatedly to measure reflexes. Rats were giving the medication until they died.

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Testing blood pressure drugs against seizures

Rats were implanted with devices to measure heart and brain activity through induced seizures without being anaesthetised. They were killed two days later, and their hearts were taken.

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Studying the effect of prolactin on the brain

Virgin rats and rats who recently gave birth had a cannula (tube) placed in their brain and were injected with medication. They were injected with prolactin after two days and killed an hour later to dissect their brains.

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Testing a surgical treatment for hypertension with diabetes

Transgenic rats were injected to develop diabetes. Their kidney nerves were surgically destroyed. They were restrained for measurements and sampling regularly. In the end, they were all killed.

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Testing a new pain medication

Rats were operated on to cause pain build-up in their hind leg. After 10 days, they were injected with different variations of a drug or just a control solution. The effect was tested by poking their hurting leg and having them balance.

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Trying to study depressants and antidepressants

Rats were kept isolated or in groups for nine weeks. They were injected with different drugs and put through behavioural tests (the notorious Forced Swim Test, among others). In the end, all were killed.

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Studying how anaesthetics affect intestine movement

Guinea pigs and rats were anaesthetised, then dissected, and then killed, in that order.

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Studying hormone sensitivity of the brain

Female rats were mated, gave birth and cared for their babies for three weeks. They had prolactin injected below the skull. They were killed, and their brains were removed.

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Harvesting rabbit and rat hearts for lab tests

NZ White rabbits and rats were killed to use their hearts in lab tests.

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Testing digestion of a pig food

Rats were fed a pig food and killed to examine their intestines.

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Testing how heart calcium metabolism is different in species

Guinea pigs and rats were decapitated, and their hearts were quickly removed to study them.

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Investigating how worms spread between lambs and calves

Lambs and calves were treated against parasites and then infected with worm eggs. Infection status was monitored. This was repeated several times before all sheep were killed.

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Using sheep to try and model how well humans will heal after dental surgery

Sheep were anaesthetised, three teeth were pulled, and the sockets were treated differently. For months, regular samples were taken from the healing wounds before all sheep were killed.

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Preclinical testing of a gene therapy for Batten disease

Sheep with and without a specific gene mutation were checked and scanned regularly for nine months. Affected sheep received gene therapy at different ages. Treated sheep were killed.

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Testing if blocking a cell signal can prevent foetal brain injury

Pregnant sheep were anaesthetised, and the foetuses fitted with electrodes. The umbilical cord was blocked for most of them, and half were treated with a test drug. Mothers and unborn lambs were killed three weeks later.

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Testing the combination of drugs for congestive heart failure treatment

Sheep were anaesthetised and subjected to surgery. Electrodes were used to pace their hearts faster while testing the effect of different drugs.

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Testing the best way to deliver gene therapy drugs

Sheep were anaesthetised. Their heads were drilled into, and their brains injected with test drugs. They were killed after three weeks.

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Testing the potency of pneumonia vaccines for sheep

Lambs were vaccinated twice, and regular blood samples were taken for 34 weeks.

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Testing the effects and wash-out of painkillers

Lambs were treated with different painkillers or not at all before tail docking and castration. Their behaviour was monitored.

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Developing “hyperimmune” milk

Sheep were injected with Covid-19 vaccines, and regular blood samples were taken. Some were mated and gave birth to sample their milk, too.

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Studying the genetics of Batten disease on chimeric lambs

Sheep foetuses were removed. After swapping cells between them, they were inserted into other ewes. Lambs born were subjected to X-rays and, at one to three years old, were bled out for dissection.

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Testing pacemaker adjustments

After causing them heart failure, sheep were surgically fitted with pacemakers and measurement devices. Those who survived the procedures were killed after six months.

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Studying methane emission and meat quality

Sheep were bred for high and low methane emissions. Almost 2,000 were put through emission measurements. Over 17,000 were slaughtered.

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Studying the effect of chemicals in normal and high blood pressure

Sheep were surgically fitted with a clip around an artery, or they served as control. Measuring devices were added before injecting different chemicals into the hearts of the conscious sheep.

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Comparing muscle repair success with and without weeks of delay

Ewes were anaesthetised, and one side of a pelvis muscle was detached. After several weeks, the muscle was repaired. Gait was evaluated before all were killed.

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Comparing different designs of dental implants

Sheep were anaesthetised. One leg bone was exposed and fitted with several implants. After 12 weeks, all were killed.

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Trying to model human brain injuries

To study changes in different areas of the brain after brain injuries. Sheep were used to study the effects of traumatic brain injuries in humans.

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Investigating intestine movements

Young pigs and NZ White rabbits were anaesthetised. A part of their intestine was pulled out far enough to record its movements and signalling.

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Harvesting rabbit and pig eyes for lab tests

The eyes of slaughtered rabbits and pigs killed for experiments were used to analyse the structure of the cornea.

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Causing sinusitis in rabbits as a model

NZ White rabbits were anaesthetised, and a sinus tract in their nose was blocked for four weeks to cause sinusitis. After another ten weeks with regular samples, they were killed.

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Testing a new vaccine

NZ White rabbits were injected with a new vaccine type several times before being killed.

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Harvesting eyes for lab tests

The eyes of pigs and rabbits killed for experiments were used to analyse the structure of the cornea.

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Testing the toxicity of some substances

Young NZ White rabbits were force-fed different doses of toxic substances. Blood samples were taken before and immediately after killing them at set time points (or to end their suffering).

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Using a new imaging technology

One NZ White rabbit and rats were killed, and their eyes were removed to use for the study.

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Measuring contractions in exposed rabbit uteruses

Pregnant rabbits were anaesthetised, and their uterus was exposed. Measurements were taken while contraction-inducing injections were given. Then they were all killed for dissection.

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Mapping wave patterns of the small intestine

NZ White rabbits were anaesthetised, their intestines were used for electrode mapping, and then they were killed.

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Studying presence of a virus in rabbits

Wild rabbits were shot, caught by dogs or trapped to test them for virus strains. The infectious solution was then fed to NZ White rabbits in a lab to kill them later.

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Harvesting rabbit colons for lab tests

NZ White rabbits were anaesthetised, a part of their intestine was cut out, and then they were killed.

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Harvesting rabbit bladders for lab tests

NZ White rabbits were anaesthetised, their bladder was cut out, and then they were killed.

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Testing an antimicrobial eye medication

NZ White rabbits were treated with Manuka honey solution in one eye and a control solution in the other eye for five days. Different values like tearing and irritation were measured.

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Using muscles of dead rabbits for imaging

Two NZ White rabbits were killed to use for detailed muscle imaging.

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Measuring jaw bone growth in young rabbits

Young rabbits were anaesthetised and had holes drilled into their jawbones to implant small pieces of metal. X-ray scans were made, and the rabbits were killed.

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Testing the welfare impact of pindone toxin

Three groups of young NZ White Rabbits received pindone-toxin pellets on different schedules. Any rabbit still alive after three weeks was killed.

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