Cattle

Cattle are one of the most used animals for science in NZ. Learn about the many ways they are used!

Thousands of cattle are used for scientific purposes in Aotearoa every year. In fact, they have been one of the most commonly used animals in NZ science since 1989!

This sadly makes sense when you consider how widely they are exploited for their milk and their bodies.

The species of cattle is simplified to cows by many people. Actually, only the mothers are called cows:

  • Heifers are female cattle before they become mothers.
  • Calves are their babies.
  • The males are referred to as bulls (or steers if castrated).
Animal agriculture:

Thousands of cattle are used in Aotearoa every year in research aimed at trying to sustain, enhance and make more money for the animal agriculture industry.

Examples include research into:

1. Decreasing their negative impact on the environment - a cow on its own won't create a huge impact but because we have bred so many cattle in NZ, their collective impact is huge!

  • Cattle have been put in respiration chambers so the amount of methane they produce can be measured. Researchers have also glued urine sensors around their vulva to try and investigate ways of reducing the amount of nitrogen produced in their urine (methane and nitrogen can create negative impacts on the environment).

2. Increasing the survival rate of calves - not for the calves themselves but so the animal agriculture industry doesn't lose as much money.

3. Learning more about the fertility of cows (i.e., trying to improve artificial insemination and reduce the rest time in-between pregnancies for cows). Again, this research is not done for the cows themselves but so they be made pregnant as often as possible so that more money can be made from their exploitation.

4. Genetic modification to try and create more desirable traits in cattle (i.e., genetically modifying cows to change the properties of their milk).

5. Diseases that cattle on farms often get such as lameness, mastitis (a common infection of the udder in dairy cows) and fungal and parasitic infections. This type of research often involves making the animals sick on purpose.

6. Ways of housing and keeping large numbers of cattle on farms (i.e., research into bedding, floor types, shelter, shade and hygiene practices).

7. Trying to find more “humane” slaughter methods (i.e., investigating what type of gas kills piglets the quickest and if stunning animals before killing them creates less of a pain response).

8. Different types of pain relief for standard procedures in the animal agriculture industry (i.e. dehorning, castration, C-section).

9. Safety tests for chemicals and animal remedies, including medications, supplements and fertilizers.

10. Increasing milk or muscle (beef) production (i.e., feeding cattle different additives and measuring how its digested).

Fistulation: Cattle with holes cut into their bodies

For decades, researchers have artificially created fistulas (also known as cannulas) in ruminant animals such as cattle, sheep and deer. These are essentially window-like holes in the side of an animal's body.

Fistulas are tubes that are created surgically to connect a cow or sheep’s stomach to her skin. The outside of the fistula is covered with a plastic cap that can be opened by scientists to remove stomach contents for analysis or introduce foreign substances.

It is a painful procedure and cows are still suffering from the intrusive surgery up to 11 days after the surgery. Learn more here.

Additional calf exploitation - the calf blood industry

Calves and calf foetuses are killed, and the blood is drained from their bodies to be used in other research.

There are two main types of calf blood products: Fetal bovine serum and New Born Calf Serum.

Fetal bovine serum is taken from foetuses that have been removed from their mothers in slaughterhouses after they have been killed. A needle is injected into the foetus’ heart, and the blood is pumped out. The foetuses have to be alive during this so that their blood comes out easier. They also have to be at least three months into the gestation period to ensure that their heart is large enough to puncture.

Newborn Calf Serum is sourced similarly; calves who are 20 days old or younger have their heart punctured and are bled to death.

These products are used as a media base and growth supplement in many different types of research.

In the news

Mycoplasma Bovis: "Researchers will measure how M. Bovis impacts infected animals and herds, including physical signs, effects on milk yield and quality, weight gain in cattle, and the duration of these effects." Read more.

Cow shock collars: "Animals wear GPS-enabled collars that identify their location relative to invisible fence lines created on a digital farm map. The animals are trained to be guided by audio prompts (beeps) from the collars when required. As an animal gets close to a virtual fence, a series of beeps alerts them to the fence boundary. If they cross the fence line, they receive a "disincentive", a mild electric shock, and are guided back inside the line." Read more.

Bobby calves: Bobby calves (male calves of dairy cows) are normally unwanted by the dairy industry. Researchers are cross-breeding dairy cows with beef breeds so that bobby calves can be killed and eaten after a year, rather than being killed as "excess" after 4 days. Read more

Climate change: AgResearch scientists have been given $10 million to develop cattle better adapted to warmer temperatures and to lower methane emissions. Read more.

Places that use cattle for science in NZ include universities such as Lincoln University (who have their own research dairy farm) and Massey University (they also have their own dairy farms), private companies such as Abacus Bio Limited and Cognosco, crown institutes such as AgResearch, industry bodies such as Dairy NZ and by other organisations and individuals.

Cattle used for science in NZ are sourced from commercial establishments, farms, public sources, breeding units and other sources.

Discover real-life examples of how cattle have been used in NZ below!
References:

Measuring thickness of blood vessel walls

Colony cats were anaesthetised, and the blood vessels under their tongue were measured with a special hand-held camera.

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Collecting faeces for lab tests

Colony cats were fed different diets for three weeks before their faeces was collected for analyses.

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Surveying caregivers on their cat feeding

New Zealand cat caregivers filled in an online survey about their household demographics and what/how they feed their cats.

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Testing new toxin in Hawke’s Bay (again)

Toxic bait traps were placed on a property in Hawke’s Bay. Cat population changes were monitored with stationary cameras.

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Assessing stress reaction to anaesthesia in cats

Cats were put through several anaesthetic infusions with six-day breaks in between. Regular saliva samples were taken 24 hours before and after, and urine was collected through a wire mesh tray.

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Trialling ready-made toxic bait

Toxic bait stations are set up in a regular pattern in an area and left for two weeks. Trial cameras and tracking tunnels monitor the effect on the population.

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Studying medical data of cat and dog CT-imaging

Medical data from cats and dogs were gathered, and animals that had been euthanised for other reasons were examined.

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Testing if a human diabetes drug might also work in cats

Research cats were placed in individual cages four times (one week each time) and injected with either a diabetes drug or a control solution. Several blood and urine samples were taken.

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Testing a cat food supplement for tooth health

Colony cats were anaesthetised, and all plaque was removed from their teeth. Four weeks later, new plaque build-up was measured.

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Sampling cats for feline immunodeficiency virus (FIV)

Buccal swabs (inside the cheek) were collected from almost 200 cats at 15 veterinary clinics.

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Testing food preferences of cats

Cats were fasted for two hours in the mornings and then presented with different foods (lamb and beef parts) to measure their consumption.

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Monitoring wild cats for a year

Wild cats are trapped and fitted with GPS collars. After one year, they are recaptured and killed.

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Trialling a lighter rifle to kill trapped wild cats

Captured wild cats were shot in the head using an air rifle and were assessed to confirm death by recording the time to loss of heartbeat.

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Testing a predictive marker for cancer survival in cats

Veterinary samples of cats with this form of cancer were analysed and compared with their remaining survival since diagnosis.

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Monitoring outdoor cat behaviour

Privately owned cats with outdoor access were fitted with camera and GPS collars for up to three days.

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Testing a diagnostic for feline coronavirus

Cats with specific symptoms were used to take blood, tissue, and fluid samples. Some blood samples were taken from control cats.

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Studying the genes for hairyness

The hairiness of male and female cattle was rated. Some animals had their rectal temperature taken repeatedly, and some were shut in a hot room for two hours to make them sweat. Eight calves were killed to take brain samples.

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Optimising fertility treatment

Young female cattle were treated with different hormonal injections to control their fertility cycle. Around ten rectal ultrasounds were done on each of them within 11 days, and several blood samples were taken in the next month. All but nine were artificially inseminated. The nine animals showed no fertility signs and were therefore useless for the study and the industry.

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Trying out a health test for breeding bulls

Breeding bulls were infected with a skin fungus that usually results in a dangerous build-up in the gall and eczema. Through a special diet, bulls can be tested for their reactions via repeated blood samples and ranked for their genetic quality.

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Testing a vaccine against miscarriage

Younger and older pregnant cattle were injected with a new vaccine against a parasite or just control solution. Skin reactions to the injection were checked, and blood samples were taken. Farmers reported miscarriages when they happened, and dead foetuses were collected if possible. If female calves were born, blood samples were taken from them, too.

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Studying Johne’s disease

Some female calves of a group were infected with bacteria causing life-threatening sickness. Poo samples were collected directly from the rectum of all calves every other month. Half were killed to be dissected at 7 months, the rest at 15 months.

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Studying Johne’s disease bacteria

Blood samples were taken from sick dairy cows who were sent to slaughter to see if their immune system was even putting up a fight. After slaughter, organ samples were taken.

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Finding a gene to increase casein in milk

Breeding bulls were evaluated on their quality by judging, breeding, and milking their daughters. No exact numbers are stated how many lived to the end of the study (5 lactations).

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Testing a new way to treat liver flukes

Young cattle were infected with liver flukes. They were treated with different medications or left untreated. Regular blood samples were taken from all animals for three weeks, and they were killed ten weeks later.

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Studying effects of stunning before slaughter

Calves and sheep were anaesthetised. Some got 70-volt shocks to the shoulder. Others had a nerve exposed and stimulated before being stunned or cutting their throat.

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Measuring brain reactions to stunning after killing

Calves were fasted for 24 hours and then anaesthetised. They were killed by their throats being cut, followed by stunning while measuring EEG/ECG (electronic heart and brain activity) and blood pressure.

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Measuring brain reactions to stunning

Calves were fasted, anaesthetised and stunned with a non-penetrative bolt while being monitored for their response. After measurements were taken, they were killed.

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Studying brain reactions to cutting blood vessels or neck tissue

Calves were anaesthetised, and their throat blood vessels exposed. While monitoring the brain“s activity (EEG) and blood pressure, either only the blood vessels or only the neck tissue was cut. The latter animals were then killed, too.

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Measuring if being slaughtered is unpleasant

Calves were fasted, anaesthetised, and killed by cutting their throat. EEG and ECG (recording electronic heart and brain activity) were measured with electrodes in the head.

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Measuring brain reactions to pain

Twenty calves were fasted and anaesthetised. Their horn buds were removed with a scoop, and pain response of the heart and brain with and without pain blockers.

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Testing mastitis treatments

Dairy cows with an udder infection were treated with different antibiotic injections. Milk samples were taken to monitor results.

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Trying to clone cows with more valuable milk protein

The genetic material of a cow foetus was manipulated and cloned into several cows. When they were less than one year old, they were injected with hormones to produce milk for sampling.

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Analysing Greyhound race injury statistics

Historical data on Greyhound races between 2014 and 2019 were analysed. Injuries and deaths were investigated.

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Using bones of euthanised shelter dog

Cadavers of dogs killed at an animal shelter were donated. Holes were drilled into leg bones and fitted with screws.

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Investigating farm dogs for four years

Working farms dogs were examined regularly for four years. Health problems and fate were recorded.

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Transplanting faeces as diarrhoea treatment

A dog with diarrhoea was treated with laxatives and warm water before a faeces sample from a healthy dog was inserted into his colon. Samples were taken before and after.

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Testing the eyes of working dogs

Eye examinations were performed on working sheep dogs. Medication for pupil dilation was given, and faeces samples were taken from most dogs.

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Analysing dog movements

Colony dogs were prepared with reflective markers and an activity collar. Their movements on a treadmill at various speeds were recorded.

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Testing the effect of diet on farm dog performance

Working dogs were fed a diet very high or very low in carbohydrates. Their blood glucose and activity were monitored three times over four days each.

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Testing a parasite treatment

After a pilot study with just one dog, six more were given an experimental parasitic treatment. Regular blood samples were taken through a catheter.

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Teaching dogs to smell out catfish

Five pet dogs were trained over several months to identify catfish and goldfish smell in water samples.

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Testing different combinations of pain meds

Eight dogs are treated with four different combinations of the test drugs in random order with two-week breaks in between. Two catheters are placed in leg veins (for drug injection and blood samples). For the test, dogs are fasted for 12 hours and anaesthetised. EEG is recorded with electrodes near the brain to measure response to electric shocks. Regular blood samples are taken for 8 hours.

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Studying medical data of cat and dog CT-imaging

Medical data from cats and dogs were gathered, and animals that had been euthanised for other reasons were examined.

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Teaching dogs to smell out koi carps (2019)

Three pet dogs were trained over several months to identify koi carb smell in water samples.

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Testing working dogs for internal parasites

Faeces and blood samples were taken from working farm dogs.

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Testing fluid management of dogs during anaesthesia

Dogs were anaesthetised. Their heart activity was measured while hey were injected with calculated amounts of fluid.

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Trying to make rat poison unattractive for dogs

Dogs were fasted overnight and then given two food bowls with different kinds of repellent on one of them.

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Surveying respiratory disease in racing Greyhounds

Laboratories sent in serum samples of dogs for analysis. Additionally, 17 racing Greyhounds were tested.

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Studying how viruses spread in fish

Fish were caught with nets and killed.

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Using fish to study the impacts of Climate Change

Fish were either bought or caught and killed so that they could be dissected.

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Overfeeding Zebrafish to learn about obesity

Normal and transgenic zebrafish were fed normally or way too much. The fish were taken out of the water for glucose tests and blood samples.

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Studying gut bacteria in fish

Nine fish were caught by underwater spearing and killed so that samples could be collected from their digestion tracts.

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Studying the sleep of sharks

Sharks were caught and kept in outside tanks. For the experiments, they weren't fed for at least 4 days, of which they spent 3 days in a measuring chamber not much bigger than the shark. Oxygen consumption and behaviour were continuously recorded for the last 24 hours.

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Infecting fish larvae as a potential model for humans

Zebrafish larvae were injected with bacteria to test how their immune system responds. This was repeated to test how well the immune system reacted and how long the effect lasts.

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Finding a “growth gene” in snappers

Snappers were caught from the wild and bred. Around eighty-thousand of their offspring were hatched and raised. At regular “grading” events, injured and deformed fish were disposed of. At just under a year old, around twenty-two-thousand were left and transported to a sea pen, where they were raised to 17.5 months old before harvesting.

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Testing how much fat you can feed fish

Fish in aquaculture were divided into groups and fed diets with different fat content. Fish were anaesthetised and faeces samples squeezed out of them.

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Testing fish for mercury contamination

Wild fish were caught, measured and frozen to be analysed.

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Measuring how stress affects fish

Fish were exposed to chronic stress for four weeks, while 57 non-stressed animals were kept as a control. Signs of stress were noted. At the end of the experiment, all were chilled to death.

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Exposing fish to herbicides

Zebrafish were kept in tanks that were contaminated with different herbicide concentrations for 10 days. They were then mated to herbicide-exposed or control fish. Both generations were tested on behavioural changes at around 4 months of age. 24 fish per treatment were killed and dissected.

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Testing supplements as protection from toxins in over 1,000 fish embryos

Embryos were harvested and kept in Petri dishes for toxicity and treatment tests. Embryos were treated with an antioxidant, a gold salt or both on the first day, and abnormal development was measured on day 5.

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Studying effects of drying rivers on mudfish

Water depth and quality were measured at 24 different sites. Several traps for fish and invertebrates were set at 8 visits, sampling and mostly killing the caught animals (including 15 individuals of the endangered Kōwaro).

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Studying bacterial spread in fish larvae

Bacteria were genetically modified and made fluorescent. Zebrafish were bred, and their larvae were infected with the bacteria. The fish were killed for analyses or used for live imaging under a microscope (and then killed, most likely).

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Investigating effects of invasive fish removal

Using fyke nets and electrofishing, fish were caught on four occasions. Fish were marked with fin clippings around one big removal event where 3 tonnes of fish were caught. In recaptures a week after each marking event, invasive fish caught were killed after the examination.

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Studying fish life cycles

Fish were sedated and their blood and ovarian tissue were sampled three times.

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Developing a model for thermal stress in humans

Guinea pig pups were lightly anaesthetised, connected to devices measuring heart activity and breathing, and wrapped in a temperature-controlling blanket. Their body temperature was either forcefully raised or dropped.

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Testing how too much fructose is bad during pregnancy

Female guinea pigs were mated; some were fed additional sugary water until birth. The pups were used for several glucose tolerance tests and X-rays. All pups were killed in the end.

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Testing a seizure medicine for preterm babies

Pregnant guinea pigs were induced to give birth early, to test medication on their pups.

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Testing the effects of sedatives

Young guinea pigs were treated with different sedatives. Their heart rate and blood pressure were measured before and during each treatment.

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Testing an refined way of anaesthesia in guinea pigs

Guinea pigs were made to inhale anaesthetic gases. Different probes were inserted and attached to the animals to measure responses.

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Studying the effect of fructose during pregnancy

Guinea pigs were fed either normally or supplemented with fruit sugar before and during pregnancy until birth. Mothers and pups were tested for glucose tolerance.

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Studying the effects of meth

Guinea pigs were injected with sedatives, caffeine, and later meth. Under anaesthesia, their livers were cut out before they were killed.

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Studying preterm birth effects on the brain

Pregnant guinea pigs were grouped to give birth normally or be induced early. The pups were put through behavioural tests and then killed for dissection.

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Studying trauma and treatment of cochlear implants

After destroying their hearing with noise, guinea pigs were fitted with cochlear implants. Treatment success was tested before they were killed.

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Testing the measurement of pupil reflexes in Alzheimer’s research

Guinea pigs were repeatedly flashed in the eyes with a phone light to record the pupil responses.

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Teaching animal researchers

A range of animals is used to teach researchers handling and experimental methods.

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Investigating mechanisms slowing down the heart rate

Young guinea pigs were injected with blood thinner before being anaesthetised and dissected.

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Testing a device to assess hearing

Young guinea pigs were anaesthetised, electrodes placed under their skin, and their brain's response to sound was measured. All guinea pigs had one ear damaged, some both ears, before all were killed.

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Studying changes in the inner ear due to inflammation

Guinea pigs were injected into the ear with bacteria. Medical scans were done after injecting chemicals, and most were killed for dissection.

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Studying the effects of medication on memory

Guinea pigs were force-fed different drugs and later tested in a water maze before some were killed. Another group was exposed to carbon dioxide and decapitated.

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Studying how anaesthetics affect intestine movement

Guinea pigs and rats were anaesthetised, then dissected, and then killed, in that order.

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Researching scoliosis genes

Transgenic mice with a specific gene defect were mated. Pregnant mice were at several time points. The unborn babies were taken, skinned, and gutted to stain their skeletons for better pictures.

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Studying hormone influences on obesity and fertility

Transgenic mice were bred to have diabetes symptoms. Together with control mice, they were fed a normal or a high caloric diet and compared for their fertility over 150 days.

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Testing a new stroke treatment

Mice were squeezed into plastic cones, and test drugs were dripped on their nose. An artificial stroke was caused by injecting a chemical into their brain. Before and after, mice had to perform behavioural tests. All were then killed to take their brains.

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Testing another new stroke treatment

Mice were operated on several times. They were made to experience an artificial stroke, were later injected with a possible treatment, and then were injected with a marker. The mice had their memory tested before they were killed.

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Testing prostate cancer drugs

Mice were anaesthetised, and their prostate was injected with cancer cells or a control solution. A 42-day treatment course started three weeks later with different treatment drugs and control solutions. In the end, all mice were killed.

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Testing a heart attack treatment

Mice were trained to eat jelly and then subjected to an artificial heart attack. They received either a new drug or a control substance. Regular heart ultrasounds were performed before they were killed and dissected.

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Studying ovary problems caused by testosterone

Transgenic mice were implanted with a capsule containing either hormones or not. Vaginal smears were done for several weeks, and serial blood tests were performed twice. In the end, all mice were killed.

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Testing an Autism treatment on transgenic mice

Mice were genetically modified to show "autism-like" symptoms. Together with control animals, they were either fed normally or with a high-zinc diet. Invasive behavioural tests were performed. At least some, likely all, were killed.

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Comparing transgenic mice and humans

Mice were anaesthetised, and their body composition was measured several times. Their grip strength and balance were tested with lab equipment. In the end, all mice were killed.

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Testing a new stroke drug for better dexterity

Transgenic mice were anaesthetised, and an artificial stroke was caused. They then received either a test drug or a control solution. After recovery, their food was limited to test their fine motor skills every other week. After two months, they were killed.

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Testing migraine drugs

Mice were injected with potential migraine drugs and anaesthetised. Ultrasound imaging of their ears was taken. One ear was rubbed with capsaicin to record the increased blood flow and compare different drugs, doses, and time points.

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Killing rats and mice for tissue

Rats and mice were anaesthetised, and their necks were broken. Their tissue was then used for research.

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Studying Alzheimer’s and stroke symptoms

One group of transgenic mice were bred and killed at either four or 12 months of age by flushing their hearts. Another group was anaesthetised and caused to have a stroke. They were killed the same way.

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Testing Alzheimer’s treatment on mice

Transgenic and “normal” mice were injected with a special protein and a chemical to mark new brain cells that may have been produced.

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Testing a new Alzheimer’s drug

Newborn mice were killed to collect brain tissue. Adult mice were used to test drug reactions. They were anaesthetised twice; to inject the drugs and to flush their hearts and take their brains.

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Testing if anaesthesia affects the sleep–wake cycle when combined with light treatment

Mice were kept on a 12-hour light/dark cycle and then switched to total darkness. During this time, they were exposed to light once, either with or without anaesthesia. The effect on their activity cycles was monitored. In the end, all were killed.

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Harvesting colon tissue for laboratory tests

Pigs were killed and their colon tissue was used for laboratory testing.

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Testing if pigs can be fed by-products from biofuel and food processing

Pigs were fed a standard diet or a diet containing by-products from other industries. They were slaughtered to compare meat growth and quality.

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Studying starch digestion in pigs to try and model humans

Pigs were fed different diets containing durum wheat or rice. Some had regular blood samples taken through a catheter. All the animals were killed at the end.

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Validating a treatment for stomach muscle dysfunctions

Pigs were anaesthetised, their bellies were opened, and electrodes were placed on their stomach walls before and after damaging some stomach tissue with heat. All pigs were killed.

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Assessing pigs as digestion models for humans

Titanium tubes were implanted into pigs’ intestines. Digested food was collected through the tube for 9 hours.

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Testing the effect of different species’ milk on brain gene expression

Piglets were fed different kinds of milk from other species for 15 days. They were killed to dissect their brains.

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Testing transmitter implantation on wild pigs

Wild pigs were captured, and three different tracking devices were fixed to or implanted into each one. After 104 days, they were tracked down and killed.

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Investigating oxygen content in the gut

Pigs were fed diets with different or no protein sources as a control. After a week, all were killed.

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Testing a UV treatment for bacterial eye infection

Pigs’ eyes were acquired for testing a new treatment, followed by tests on live mice, most of whom were killed.

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Testing the effect of different species’ milk on protein digestion

Piglets were fed different kinds of milk from other species for 15 days. They were killed to dissect their stomachs.

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Testing the strain on a temporary surgical implant

A surgical implant was screwed to a severed pig head, and measurements were taken via laser scanning.

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Investigating intestine movements

Young pigs and NZ White rabbits were anaesthetised. A part of their intestine was pulled out far enough to record its movements and signalling.

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Harvesting rabbit and pig eyes for lab tests

The eyes of slaughtered rabbits and pigs killed for experiments were used to analyse the structure of the cornea.

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Harvesting eyes for lab tests

The eyes of pigs and rabbits killed for experiments were used to analyse the structure of the cornea.

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Testing a treatment for stomach muscle dysfunctions

Pigs were anaesthetised, their bellies were opened, and electrodes were placed on their stomach walls before and after damaging some stomach tissue with heat. All pigs were killed.

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Testing how electric stimulation affects stomach movements

Nine pigs were anaesthetised, their bellies were opened, and electrodes were placed on the stomach walls. Electric stimulation tests were performed before the pigs were killed.

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Studying food intake in an autism "model"

Pregnant rats were injected to cause autism-like symptoms in their babies. The male babies were put through behavioural tests with control animals as well as a feeding study. Some were fasted for 16 hours and killed by flushing their hearts.

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Testing the safety of a probiotic supplement

Rats were force-fed with a probiotic solution or control solution. After two weeks, they were killed for dissection.

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Studying the effects of obesity and mussel powder on bone health

Rats were kept alone and fed different diets containing normal or high amounts of fat or sugar. Some were supplemented with Greenshell mussel powder. X-rays were done, and some rats' ovaries were removed. Ultimately, all were killed by bleeding them out.

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Testing if a brain region controls memory

Some rats had their brains surgically heat-damaged. Electrodes were implanted to monitor brain activity and to stimulate the damaged parts with light. Rats then had to re-perform memory tests to see the impact before all were killed.

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Studying the communication between inner ear and brain

Rats were anaesthetised, and their inner ear was partly removed, before parts of their brains were stimulated with wire electrodes. Reactions of the rat's eyes and brain were recorded. In the end, all rats were killed.

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Testing a connection between tinnitus and brain function

Rats were anaesthetised and one ear was damaged with noise. Rats were taught that silence meant they would get electric shocks and freeze in fear. This was a test for tinnitus.

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Testing diabetic rats’ hearts

Genetically diabetic fat rats and their lean littermates were anaesthetised. Ultrasound images were made from their hearts. All were killed and dissected.

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Testing the effect of diabetes on pregnancy hormones

Rats were anaesthetised, and their brain was accessed through the mouth to measure reactions to stimulation. In the end, all rats were killed.

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Trying to study schizophrenia

Half of a group of pregnant rats were injected to affect their babies' brain development. Some pups were allowed to grow up to test their behaviour.

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Releasing a rat family into the wild to see where they go

A wild-caught rat was mated, fed a fluorescent dye, and released again with her pups. Traps, tunnels, and cameras were set up to track and eventually recapture the animals, which did not work 100%.

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Comparing digestion of sheep versus cow milk yoghurt

Rats were fed cow milk, sheep milk, cow yoghurt or sheep yoghurt for two weeks. They were gassed with CO2 before their necks were broken.

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Testing a rat poison that is unattractive for dogs

Rats were kept in small cages and fasted overnight. Then, they were given two food trays. One of them was scented with lion, tiger, or dog faeces.

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Comparing digestion of sheep versus cow milk

Rats were fed a test diet containing beef protein and received cow milk or sheep milk instead of drinking water. After 28 days, all were killed.

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Testing medication after heart attack

Rats were anaesthetised, and their hearts were exposed. A heart attack was simulated by blocking an artery. Some had dye injected into their brains, and some were injected with test drugs. All animals were killed.

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Testing a medicine against drug addiction

Mice's tails were dunked in hot water. Rats were put through cycles of drug-seeking and withdrawal using self-injection of cocaine. Other rats were injected, and their behaviour was tested (with the notorious Forces Swim Test, among others).

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Studying cell death in breast tissue

Mother rats had some of their newborns taken away, and several of her teats were sealed off after two weeks. They were killed after different waiting times to analyse the dying tissue.

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Testing out cloning as a breeding tool

Cloned embryos were implanted into 17 mother sheep but all the pregnancies failed.

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Undernourishing sheep to test glucose metabolism of their babies

Sheep were fed normally or only every three days. Some were killed during pregnancy. Born lambs were subjected to repeated glucose tolerance tests and killed.

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Testing a medication for vocal chord injuries

Sheep were anaesthetised, tissue samples were taken from their vocal folds, and most were treated with different medications. All were killed after one month.

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Testing if pre-term birth injections compromise glucose metabolism

Pre-term and full-term lambs were subjected to glucose tests, regular blood samples and a constant glucose infusion. Some were killed at four weeks, the rest at one-year-old.

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Studying the effect of Huntington’s disease on ageing

Transgenic sheep were produced through breeding, and blood samples were taken.

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Testing a first aide drug for severe bleeding

Sheep were anaesthetised and 30% of their blood was drained. After being treated with different medications, the drained blood was returned, and the sheep were killed.

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Studying ear posture while in pain

Lambs had their tails docked with a tight rubber band without pain relief. Their pain behaviour was recorded.

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Teaching animal researchers

A range of animals is used to teach researchers handling and experimental methods.

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Training students in surgical procedures

Pigs and sheep were used for training future surgeons.

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Teaching students surgical techniques

Sheep and pigs were used and killed in teaching students.

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Studying effects of stunning before slaughter

Calves and sheep were anaesthetised. Some got 70-volt shocks to the shoulder. Others had a nerve exposed and stimulated before being stunned or cutting their throat.

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Investigating intestine movements

Young pigs and NZ White rabbits were anaesthetised. A part of their intestine was pulled out far enough to record its movements and signalling.

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Harvesting rabbit and pig eyes for lab tests

The eyes of slaughtered rabbits and pigs killed for experiments were used to analyse the structure of the cornea.

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Causing sinusitis in rabbits as a model

NZ White rabbits were anaesthetised, and a sinus tract in their nose was blocked for four weeks to cause sinusitis. After another ten weeks with regular samples, they were killed.

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Testing a new vaccine

NZ White rabbits were injected with a new vaccine type several times before being killed.

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Harvesting eyes for lab tests

The eyes of pigs and rabbits killed for experiments were used to analyse the structure of the cornea.

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Testing the toxicity of some substances

Young NZ White rabbits were force-fed different doses of toxic substances. Blood samples were taken before and immediately after killing them at set time points (or to end their suffering).

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Using a new imaging technology

One NZ White rabbit and rats were killed, and their eyes were removed to use for the study.

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Measuring contractions in exposed rabbit uteruses

Pregnant rabbits were anaesthetised, and their uterus was exposed. Measurements were taken while contraction-inducing injections were given. Then they were all killed for dissection.

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Mapping wave patterns of the small intestine

NZ White rabbits were anaesthetised, their intestines were used for electrode mapping, and then they were killed.

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Studying presence of a virus in rabbits

Wild rabbits were shot, caught by dogs or trapped to test them for virus strains. The infectious solution was then fed to NZ White rabbits in a lab to kill them later.

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Harvesting rabbit colons for lab tests

NZ White rabbits were anaesthetised, a part of their intestine was cut out, and then they were killed.

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Harvesting rabbit bladders for lab tests

NZ White rabbits were anaesthetised, their bladder was cut out, and then they were killed.

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Testing an antimicrobial eye medication

NZ White rabbits were treated with Manuka honey solution in one eye and a control solution in the other eye for five days. Different values like tearing and irritation were measured.

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Using muscles of dead rabbits for imaging

Two NZ White rabbits were killed to use for detailed muscle imaging.

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Measuring jaw bone growth in young rabbits

Young rabbits were anaesthetised and had holes drilled into their jawbones to implant small pieces of metal. X-ray scans were made, and the rabbits were killed.

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Testing the welfare impact of pindone toxin

Three groups of young NZ White Rabbits received pindone-toxin pellets on different schedules. Any rabbit still alive after three weeks was killed.

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