Rats (and mice) are often the animals of choice to try and model human conditions, treatment and body functions - even though we know that using animals to try and model people fails over 90% of the time.

Mice and rats are commonly used due to their small size, low maintenance (i.e., they are easy to house and care for), short life cycle, and ability to breed quickly (allowing large numbers to be generated for studies).

They also share many of our genes.1 However, that's not surprising. Even cats share many of our genes.2

Rats are used is some of the most gruesome experiments that involve things like force-feeding, poisoning, oxygen deprivation and invasive surgery.

Rats in NZ have been used for:
  • Drug research, including safety and efficiency testing.
  • Teaching purposes in schools, mainly dissections.
  • Investigating ways of killing wild rats. (i.e., research into effective poisons and traps).
  • Medical research, including research into the development of severe diseases (i.e., stroke, diabetes, cancer), modelling neurological disorders (i.e., Schizophrenia, Autism), modelling mood disorders (i.e., example, anxiety, and depression) and research into tinnitus.
  • Drug addiction research including research into the development and suppression of addictions and the impacts of drug use.
  • Basic biological research, including research into digestion, aging, nerve damage, obesity and how the brain functions.

Places that use rats for science in NZ include crown research institutes, private companies and universities. In fact, most universities have their own breeding colonies. Otago University states on its website that they have a dedicated rat testing room in the Behavioural Phenotyping Unit.3

Rats used for science in NZ are sourced from breeding units, public or commercial sources, the wild (captured) and they are imported from overseas.

Discover real-life examples of how rats have been used in NZ below!

Measuring thickness of blood vessel walls

Colony cats were anaesthetised, and the blood vessels under their tongue were measured with a special hand-held camera.

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Collecting faeces for lab tests

Colony cats were fed different diets for three weeks before their faeces was collected for analyses.

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Surveying caregivers on their cat feeding

New Zealand cat caregivers filled in an online survey about their household demographics and what/how they feed their cats.

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Testing new toxin in Hawke’s Bay (again)

Toxic bait traps were placed on a property in Hawke’s Bay. Cat population changes were monitored with stationary cameras.

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Assessing stress reaction to anaesthesia in cats

Cats were put through several anaesthetic infusions with six-day breaks in between. Regular saliva samples were taken 24 hours before and after, and urine was collected through a wire mesh tray.

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Trialling ready-made toxic bait

Toxic bait stations are set up in a regular pattern in an area and left for two weeks. Trial cameras and tracking tunnels monitor the effect on the population.

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Studying medical data of cat and dog CT-imaging

Medical data from cats and dogs were gathered, and animals that had been euthanised for other reasons were examined.

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Testing if a human diabetes drug might also work in cats

Research cats were placed in individual cages four times (one week each time) and injected with either a diabetes drug or a control solution. Several blood and urine samples were taken.

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Testing a cat food supplement for tooth health

Colony cats were anaesthetised, and all plaque was removed from their teeth. Four weeks later, new plaque build-up was measured.

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Sampling cats for feline immunodeficiency virus (FIV)

Buccal swabs (inside the cheek) were collected from almost 200 cats at 15 veterinary clinics.

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Testing food preferences of cats

Cats were fasted for two hours in the mornings and then presented with different foods (lamb and beef parts) to measure their consumption.

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Monitoring wild cats for a year

Wild cats are trapped and fitted with GPS collars. After one year, they are recaptured and killed.

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Trialling a lighter rifle to kill trapped wild cats

Captured wild cats were shot in the head using an air rifle and were assessed to confirm death by recording the time to loss of heartbeat.

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Testing a predictive marker for cancer survival in cats

Veterinary samples of cats with this form of cancer were analysed and compared with their remaining survival since diagnosis.

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Monitoring outdoor cat behaviour

Privately owned cats with outdoor access were fitted with camera and GPS collars for up to three days.

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Testing a diagnostic for feline coronavirus

Cats with specific symptoms were used to take blood, tissue, and fluid samples. Some blood samples were taken from control cats.

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Investigating how worms spread between lambs and calves

Lambs and calves were infected with worm eggs to see if parasites from cattle can infect sheep.

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Testing a fertility treatment

Cows with fertility problems were treated with hormones for ten days or left untreated. All were then artificially inseminated.

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Testing a supplement for more calcium intake

Pregnant cows were fed with or without a specific feed additive. After birth, all were fed the same diet. Blood samples were taken in the weeks around birth.

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Checking endometritis rates in dairy herds

Some cows in several dairy herds were selected, and their vaginal discharge was measured and sampled. They were then impregnated as per standard agricultural practice.

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Comparing weight gain with different diets

Newborn calves were raised on various milk, pasture, and pellets combinations. They were slaughtered at two years old, and their “quality” was measured.

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Testing pain meds for disbudding

The horn buds of young calves who were slightly sedated were burned off with a hot iron. Half of them received pain meds beforehand, and blood samples were taken before and after. After 24 hours, the second group received pain meds, too.

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Studying Ikeda infection and treatment

Regular blood samples were taken from cows on three different farms. Only some were treated against the parasite. Milk production and fertility were recorded.

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Studying diarrhea infection

New mother cows had blood samples taken. If the sample was negative for a diarrhoea virus, they were sampled again after a while.

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Studying genes for body size

Udders of dairy cows were slightly cut with a scalpel, and a tissue sample was taken with a biopsy needle.

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Testing toxicity of a potential new fertiliser

Pregnant cows were fed a toxic ingredient to see if it is toxic.

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Studying stress around birth

Blood samples were taken from cows with a high or low risk of health concerns after birth.

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Studying genetics of a missing tail

Cloned foetuses were implanted into female cattle to research taillessness. Some of them were killed during pregnancy to examine the foetuses. One tailless animal was killed at age 4, and one of the cloned calves died after birth.

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Studying Ikeda (parasite) infection

Blood samples were taken from dairy cows and their calves directly after birth before separating them. After four months, the calves were sampled again.

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Studying Osteoporosis in cows

Young female cattle who had given birth for the first time were sent to slaughter. Half of them had broken bones for between 2 and 10 days. Blood samples were taken before slaughter, and bones were collected after.

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Testing a tuberculosis vaccine

Male calves were infected with bacteria. Two groups were vaccinated before or after the infection, and the third group was left untreated. All were killed and dissected 13 weeks later.

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Testing feeding plans for better immunity after calving

Pregnant cows were fed either a bit too much or a bit too little. Blood samples were taken before, at, and after calving. Researchers also took liver samples with a 20-cm biopsy needle and vaginal samples with a small brush and a scoop at these dates, except for the date of birth.

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Analysing Greyhound race injury statistics

Historical data on Greyhound races between 2014 and 2019 were analysed. Injuries and deaths were investigated.

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Using bones of euthanised shelter dogs

Cadavers of dogs killed at an animal shelter were donated. Holes were drilled into leg bones and fitted with screws.

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Investigating farm dogs for four years

Working farms dogs were examined regularly for four years. Health problems and fate were recorded.

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Transplanting faeces as diarrhoea treatment

A dog with diarrhoea was treated with laxatives and warm water before a faeces sample from a healthy dog was inserted into his colon. Samples were taken before and after.

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Testing the eyes of working dogs

Eye examinations were performed on working sheep dogs. Medication for pupil dilation was given, and faeces samples were taken from most dogs.

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Analysing dog movements

Colony dogs were prepared with reflective markers and an activity collar. Their movements on a treadmill at various speeds were recorded.

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Testing the effect of diet on farm dog performance

Working dogs were fed a diet very high or very low in carbohydrates. Their blood glucose and activity were monitored three times over four days each.

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Testing a parasite treatment

After a pilot study with just one dog, six more were given an experimental parasitic treatment. Regular blood samples were taken through a catheter.

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Teaching dogs to smell out catfish

Five pet dogs were trained over several months to identify catfish and goldfish smell in water samples.

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Testing different combinations of pain meds

Eight dogs are treated with four different combinations of the test drugs in random order with two-week breaks in between. Two catheters are placed in leg veins (for drug injection and blood samples). For the test, dogs are fasted for 12 hours and anaesthetised. EEG is recorded with electrodes near the brain to measure response to electric shocks. Regular blood samples are taken for 8 hours.

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Studying medical data of cat and dog CT-imaging

Medical data from cats and dogs were gathered, and animals that had been euthanised for other reasons were examined.

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Teaching dogs to smell out koi carps (2019)

Three pet dogs were trained over several months to identify koi carb smell in water samples.

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Testing working dogs for internal parasites

Faeces and blood samples were taken from working farm dogs.

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Testing fluid management of dogs during anaesthesia

Dogs were anaesthetised. Their heart activity was measured while hey were injected with calculated amounts of fluid.

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Trying to make rat poison unattractive for dogs

Dogs were fasted overnight and then given two food bowls with different kinds of repellent on one of them.

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Surveying respiratory disease in racing Greyhounds

Laboratories sent in serum samples of dogs for analysis. Additionally, 17 racing Greyhounds were tested.

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Studying how viruses spread in fish

Fish were caught with nets and killed.

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Using fish to study the impacts of Climate Change

Fish were either bought or caught and killed so that they could be dissected.

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Overfeeding Zebrafish to learn about obesity

Normal and transgenic zebrafish were fed normally or way too much. The fish were taken out of the water for glucose tests and blood samples.

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Studying gut bacteria in fish

Nine fish were caught by underwater spearing and killed so that samples could be collected from their digestion tracts.

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Studying the sleep of sharks

Sharks were caught and kept in outside tanks. For the experiments, they weren't fed for at least 4 days, of which they spent 3 days in a measuring chamber not much bigger than the shark. Oxygen consumption and behaviour were continuously recorded for the last 24 hours.

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Infecting fish larvae as a potential model for humans

Zebrafish larvae were injected with bacteria to test how their immune system responds. This was repeated to test how well the immune system reacted and how long the effect lasts.

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Finding a “growth gene” in snappers

Snappers were caught from the wild and bred. Around eighty-thousand of their offspring were hatched and raised. At regular “grading” events, injured and deformed fish were disposed of. At just under a year old, around twenty-two-thousand were left and transported to a sea pen, where they were raised to 17.5 months old before harvesting.

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Testing how much fat you can feed fish

Fish in aquaculture were divided into groups and fed diets with different fat content. Fish were anaesthetised and faeces samples squeezed out of them.

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Testing fish for mercury contamination

Wild fish were caught, measured and frozen to be analysed.

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Measuring how stress affects fish

Fish were exposed to chronic stress for four weeks, while 57 non-stressed animals were kept as a control. Signs of stress were noted. At the end of the experiment, all were chilled to death.

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Exposing fish to herbicides

Zebrafish were kept in tanks that were contaminated with different herbicide concentrations for 10 days. They were then mated to herbicide-exposed or control fish. Both generations were tested on behavioural changes at around 4 months of age. 24 fish per treatment were killed and dissected.

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Testing supplements as protection from toxins in over 1,000 fish embryos

Embryos were harvested and kept in Petri dishes for toxicity and treatment tests. Embryos were treated with an antioxidant, a gold salt or both on the first day, and abnormal development was measured on day 5.

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Studying effects of drying rivers on mudfish

Water depth and quality were measured at 24 different sites. Several traps for fish and invertebrates were set at 8 visits, sampling and mostly killing the caught animals (including 15 individuals of the endangered Kōwaro).

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Studying bacterial spread in fish larvae

Bacteria were genetically modified and made fluorescent. Zebrafish were bred, and their larvae were infected with the bacteria. The fish were killed for analyses or used for live imaging under a microscope (and then killed, most likely).

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Investigating effects of invasive fish removal

Using fyke nets and electrofishing, fish were caught on four occasions. Fish were marked with fin clippings around one big removal event where 3 tonnes of fish were caught. In recaptures a week after each marking event, invasive fish caught were killed after the examination.

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Studying fish life cycles

Fish were sedated and their blood and ovarian tissue were sampled three times.

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Questioning vets on pain management

Veterinarians completed a questionnaire regarding pain and pain management in rabbits and guinea pigs.

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Testing nerve reactions to electric stimulation

Young guinea pigs were bled to death. Specific nerves were removed and experimented on.

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Studying short-sightedness

Guinea pigs had one eye covered for two weeks before increasing their eye pressure surgically and kill them.

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Measuring nerve reactions to gases

Guinea pigs were anaesthetised, and their windpipe and blood vessels were cannulated. They were exposed to different gases and cyanide while measuring breathing and nerve activity.

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Studying how anaesthesia affects breathing and heart

Guinea pigs were put in airtight chambers to test the effects of low oxygen. Some were surgically fitted with tubing so blood samples could be taken during the tests. In the end, all were killed.

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Studying compensation after inner ear destruction

Guinea pigs were anaesthetised, and their inner ear was destroyed with a drill. Videos were made for over two days of their balance recovery.

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Killing pregnant guinea pigs for tissue cultures

Pregnant guinea pigs were anaesthetised at specific time points of their pregnancy, including during labour, to then remove the uterus for tissue culture experiments.

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Testing how oxytocin injections cause contractions

Pregnant guinea pigs were surgically implanted with electrodes on or near the uterus. They were injected with oxytocin in specific doses and timeframes, and the effects were measured.

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Harvesting testicles for studies

Male guinea pigs were killed with CO2 to remove their testicles for experiments.

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Testing a new tuberculosis vaccine

A new tuberculosis vaccine was tested by injecting guinea pigs with the bacteria, either with or without prior vaccination. After several weeks, all were killed.

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Testing how heart calcium metabolism is different in species

Guinea pigs and rats were decapitated, and their hearts were quickly removed to study them.

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Testing influences on the rate of a dissected heart

Albino guinea pigs were anaesthetised and dissected before they were killed.

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Confirming the correlation of energy storage and stress in the inner ear fluid

Guinea pigs were anaesthetised and artificially ventilated. Ear fluid samples were taken through a hole in the skull, while exposing them to noise or lack of oxygen.

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Comparing natural and genetically altered tuberculosis strains

Guinea pigs were infected with tuberculosis strains after being injected with bacterial proteins beforehand. In the end, all guinea pigs were killed and dissected.

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Comparing effects of anaesthetics on breathing and heart rate

Guinea pigs, rabbits and rats were anaesthetised, and a tube was put into their windpipe through the neck. They were made to breathe anaesthetic gas.

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Testing motherly instinct towards foster pups

Mice of different “status” (never pregnant, pregnant, new mums) were made to rescue foster pups they didn’t know from several places. Transgenic mice lacking a prolactin brain receptor were tested for anxiety behaviour in the standard elevated-plus-maze.

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Testing new cancer vaccines

Mice were injected with cancer cells, followed by one of several potential vaccines. They were killed when tumours reached a certain size, or it became otherwise inhumane to keep them alive.

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Testing if pregnancy affects the healing of teeth

Pregnant and non-pregnant mice were taken. After the pregnant ones gave birth, all mice were anaesthetised. Some of their teeth were forcefully moved. Two weeks later, all were killed.

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Testing the effect of obesity during pregnancy

Pregnant mice were fed high-fat diets and had their babies removed via caesarean section. The foetuses were decapitated. Other newborn mice were separated to record their calls.

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Testing how motherly mice are

Transgenic mice were compared to “normal” mice on rescuing pups and how they care for their babies. They were mated, gave birth, and then put through behavioural tests involving newborn mice. After a few weeks, they were killed.

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Testing a medicine against drug addiction

Mice's tails were dunked in hot water. Rats were put through cycles of drug-seeking and withdrawal using self-injection of cocaine. Other rats were injected, and their behaviour was tested (with the notorious Forces Swim Test, among others).

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Teaching animal researchers

A range of animals is used to teach researchers handling and experimental methods.

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Testing the viability of xenotransplantation using islet capsules

Newborn piglets were killed for their pancreas. Encapsulated pancreas cells were then implanted into young mice, who were killed later to retrieve the capsules.

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Harvesting colon tissue for laboratory tests

Pigs were killed and their colon tissue was used for laboratory testing.

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Testing if pigs can be fed by-products from biofuel and food processing

Pigs were fed a standard diet or a diet containing by-products from other industries. They were slaughtered to compare meat growth and quality.

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Studying starch digestion in pigs to try and model humans

Pigs were fed different diets containing durum wheat or rice. Some had regular blood samples taken through a catheter. All the animals were killed at the end.

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Validating a treatment for stomach muscle dysfunctions

Pigs were anaesthetised, their bellies were opened, and electrodes were placed on their stomach walls before and after damaging some stomach tissue with heat. All pigs were killed.

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Assessing pigs as digestion models for humans

Titanium tubes were implanted into pigs’ intestines. Digested food was collected through the tube for 9 hours.

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Testing the effect of different species’ milk on brain gene expression

Piglets were fed different kinds of milk from other species for 15 days. They were killed to dissect their brains.

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Testing transmitter implantation on wild pigs

Wild pigs were captured, and three different tracking devices were fixed to or implanted into each one. After 104 days, they were tracked down and killed.

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Investigating oxygen content in the gut

Pigs were fed diets with different or no protein sources as a control. After a week, all were killed.

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Testing a UV treatment for bacterial eye infection

Pigs’ eyes were acquired for testing a new treatment, followed by tests on live mice, most of whom were killed.

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Testing the effect of different species’ milk on protein digestion

Piglets were fed different kinds of milk from other species for 15 days. They were killed to dissect their stomachs.

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Testing the strain on a temporary surgical implant

A surgical implant was screwed to a severed pig head, and measurements were taken via laser scanning.

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Investigating intestine movements

Young pigs and NZ White rabbits were anaesthetised. A part of their intestine was pulled out far enough to record its movements and signalling.

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Harvesting rabbit and pig eyes for lab tests

The eyes of slaughtered rabbits and pigs killed for experiments were used to analyse the structure of the cornea.

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Harvesting eyes for lab tests

The eyes of pigs and rabbits killed for experiments were used to analyse the structure of the cornea.

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Testing a treatment for stomach muscle dysfunctions

Pigs were anaesthetised, their bellies were opened, and electrodes were placed on their stomach walls before and after damaging some stomach tissue with heat. All pigs were killed.

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Testing how electric stimulation affects stomach movements

Nine pigs were anaesthetised, their bellies were opened, and electrodes were placed on the stomach walls. Electric stimulation tests were performed before the pigs were killed.

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Studying food intake in an autism "model"

Pregnant rats were injected to cause autism-like symptoms in their babies. The male babies were put through behavioural tests with control animals as well as a feeding study. Some were fasted for 16 hours and killed by flushing their hearts.

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Testing the safety of a probiotic supplement

Rats were force-fed with a probiotic solution or control solution. After two weeks, they were killed for dissection.

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Studying the effects of obesity and mussel powder on bone health

Rats were kept alone and fed different diets containing normal or high amounts of fat or sugar. Some were supplemented with Greenshell mussel powder. X-rays were done, and some rats' ovaries were removed. Ultimately, all were killed by bleeding them out.

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Testing if a brain region controls memory

Some rats had their brains surgically heat-damaged. Electrodes were implanted to monitor brain activity and to stimulate the damaged parts with light. Rats then had to re-perform memory tests to see the impact before all were killed.

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Studying the communication between inner ear and brain

Rats were anaesthetised, and their inner ear was partly removed, before parts of their brains were stimulated with wire electrodes. Reactions of the rat's eyes and brain were recorded. In the end, all rats were killed.

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Testing a connection between tinnitus and brain function

Rats were anaesthetised and one ear was damaged with noise. Rats were taught that silence meant they would get electric shocks and freeze in fear. This was a test for tinnitus.

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Testing diabetic rats’ hearts

Genetically diabetic fat rats and their lean littermates were anaesthetised. Ultrasound images were made from their hearts. All were killed and dissected.

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Testing the effect of diabetes on pregnancy hormones

Rats were anaesthetised, and their brain was accessed through the mouth to measure reactions to stimulation. In the end, all rats were killed.

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Trying to study schizophrenia

Half of a group of pregnant rats were injected to affect their babies' brain development. Some pups were allowed to grow up to test their behaviour.

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Releasing a rat family into the wild to see where they go

A wild-caught rat was mated, fed a fluorescent dye, and released again with her pups. Traps, tunnels, and cameras were set up to track and eventually recapture the animals, which did not work 100%.

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Comparing digestion of sheep versus cow milk yoghurt

Rats were fed cow milk, sheep milk, cow yoghurt or sheep yoghurt for two weeks. They were gassed with CO2 before their necks were broken.

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Testing a rat poison that is unattractive for dogs

Rats were kept in small cages and fasted overnight. Then, they were given two food trays. One of them was scented with lion, tiger, or dog faeces.

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Comparing digestion of sheep versus cow milk

Rats were fed a test diet containing beef protein and received cow milk or sheep milk instead of drinking water. After 28 days, all were killed.

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Testing medication after heart attack

Rats were anaesthetised, and their hearts were exposed. A heart attack was simulated by blocking an artery. Some had dye injected into their brains, and some were injected with test drugs. All animals were killed.

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Testing a medicine against drug addiction

Mice's tails were dunked in hot water. Rats were put through cycles of drug-seeking and withdrawal using self-injection of cocaine. Other rats were injected, and their behaviour was tested (with the notorious Forces Swim Test, among others).

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Studying cell death in breast tissue

Mother rats had some of their newborns taken away, and several of her teats were sealed off after two weeks. They were killed after different waiting times to analyse the dying tissue.

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Testing out cloning as a breeding tool

Cloned embryos were implanted into 17 mother sheep but all the pregnancies failed.

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Undernourishing sheep to test glucose metabolism of their babies

Sheep were fed normally or only every three days. Some were killed during pregnancy. Born lambs were subjected to repeated glucose tolerance tests and killed.

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Testing a medication for vocal chord injuries

Sheep were anaesthetised, tissue samples were taken from their vocal folds, and most were treated with different medications. All were killed after one month.

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Testing if pre-term birth injections compromise glucose metabolism

Pre-term and full-term lambs were subjected to glucose tests, regular blood samples and a constant glucose infusion. Some were killed at four weeks, the rest at one-year-old.

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Studying the effect of Huntington’s disease on ageing

Transgenic sheep were produced through breeding, and blood samples were taken.

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Testing a first aide drug for severe bleeding

Sheep were anaesthetised and 30% of their blood was drained. After being treated with different medications, the drained blood was returned, and the sheep were killed.

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Studying ear posture while in pain

Lambs had their tails docked with a tight rubber band without pain relief. Their pain behaviour was recorded.

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Teaching animal researchers

A range of animals is used to teach researchers handling and experimental methods.

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Training students in surgical procedures

Pigs and sheep were used for training future surgeons.

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Teaching students surgical techniques

Sheep and pigs were used and killed in teaching students.

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Studying effects of stunning before slaughter

Calves and sheep were anaesthetised. Some got 70-volt shocks to the shoulder. Others had a nerve exposed and stimulated before being stunned or cutting their throat.

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Investigating intestine movements

Young pigs and NZ White rabbits were anaesthetised. A part of their intestine was pulled out far enough to record its movements and signalling.

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Harvesting rabbit and pig eyes for lab tests

The eyes of slaughtered rabbits and pigs killed for experiments were used to analyse the structure of the cornea.

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Causing sinusitis in rabbits as a model

NZ White rabbits were anaesthetised, and a sinus tract in their nose was blocked for four weeks to cause sinusitis. After another ten weeks with regular samples, they were killed.

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Testing a new vaccine

NZ White rabbits were injected with a new vaccine type several times before being killed.

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Harvesting eyes for lab tests

The eyes of pigs and rabbits killed for experiments were used to analyse the structure of the cornea.

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Testing the toxicity of some substances

Young NZ White rabbits were force-fed different doses of toxic substances. Blood samples were taken before and immediately after killing them at set time points (or to end their suffering).

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Using a new imaging technology

One NZ White rabbit and rats were killed, and their eyes were removed to use for the study.

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Measuring contractions in exposed rabbit uteruses

Pregnant rabbits were anaesthetised, and their uterus was exposed. Measurements were taken while contraction-inducing injections were given. Then they were all killed for dissection.

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Mapping wave patterns of the small intestine

NZ White rabbits were anaesthetised, their intestines were used for electrode mapping, and then they were killed.

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Studying presence of a virus in rabbits

Wild rabbits were shot, caught by dogs or trapped to test them for virus strains. The infectious solution was then fed to NZ White rabbits in a lab to kill them later.

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Harvesting rabbit colons for lab tests

NZ White rabbits were anaesthetised, a part of their intestine was cut out, and then they were killed.

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Harvesting rabbit bladders for lab tests

NZ White rabbits were anaesthetised, their bladder was cut out, and then they were killed.

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Testing an antimicrobial eye medication

NZ White rabbits were treated with Manuka honey solution in one eye and a control solution in the other eye for five days. Different values like tearing and irritation were measured.

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Using muscles of dead rabbits for imaging

Two NZ White rabbits were killed to use for detailed muscle imaging.

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Measuring jaw bone growth in young rabbits

Young rabbits were anaesthetised and had holes drilled into their jawbones to implant small pieces of metal. X-ray scans were made, and the rabbits were killed.

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Testing the welfare impact of pindone toxin

Three groups of young NZ White Rabbits received pindone-toxin pellets on different schedules. Any rabbit still alive after three weeks was killed.

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